Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen ofParacoccidioides brasiliensiswith monoclonal antibodies
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Production and Characterization of Monoclonal Antibodies Recognizing a Common 57-kDa Antigen of Leishmania Species
Background: The therapy of leishmania infection is difficult and each year 1.5 million new cases of cutaneous leishmaniasis and 500,000 new cases of visceral leishmaniasis are estimated, therefore, there is a need for an effective vaccine. Monoclonal antibody (mAb) is one of the suitable methods for isolation and purification of leishmania antigens. In this report, we produced several mAb aga...
متن کاملproduction and characterization of monoclonal antibodies recognizing a common 57-kda antigen of leishmania species
background: the therapy of leishmania infection is difficult and each year 1.5 million new cases of cutaneous leishmaniasis and 500,000 new cases of visceral leishmaniasis are estimated, therefore, there is a need for an effective vaccine. monoclonal antibody (mab) is one of the suitable methods for isolation and purification of leishmania antigens. in this report, we produced several mab again...
متن کاملHuman parathyroid antigen: characterization and localization with monoclonal antibodies.
A cell surface antigen on human parathyroid cells was identified by monoclonal antibodies. The antigen, called parathyroid antigen (PTA), is found on both of two major polypeptides (190 kDa and 160 kDa) apparently associated exclusively with parathyroid cells. To determine whether PTA was a suitable target for in vivo imaging, 125I-labeled anti-PTA was injected into nude mice bearing human para...
متن کاملSpecific and cross-reactive monoclonal antibodies to the 89-kDa antigen of Opisthorchis viverrini.
We previously reported the production of monoclonal antibodies (MAbs) to somatic (Billings et aI, 1990) and excretory-secretory (Amornpunt et aI, 1991) antigens of Opisthorchis viverrini. The characteristics of the antigens to which these MAbs reacted are summarized as shown in Table I. It was not surprising to note that all MAbs to the worm metabolic products were directed against the 89-kDa c...
متن کاملProduction and Characterization of Murine Monoclonal Antibodies to Leishmania Gp63 Antigen
Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...
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ژورنال
عنوان ژورنال: Medical Mycology
سال: 1996
ISSN: 1369-3786,1460-2709
DOI: 10.1080/02681219680000301